TY  - JOUR
A1  -  Hernandez-Resendiz Sauri
A1  -  Vilskersts Reinis
A1  -  Aluja David
A1  -  Andreadou Ioanna
A1  -  Bencsik Péter
A1  -  Dambrova Maija
A1  -  Efentakis Panagiotis
A1  -  Gao Fei
A1  -  Giricz Zoltán
A1  -  Inserte Javier
A1  -  Kelly-Laubscher Roisin
A1  -  Kiss Attila
A1  -  Krieg Thomas
A1  -  Kwak Brenda R
A1  -  Lecour Sandrine
A1  -  Lopaschuk Gary
A1  -  M?czewski Micha?
A1  -  Waszkiewicz Micha?
A1  -  Okni?ska Marta
A1  -  Pagliaro Pasquale
A1  -  Podesser Bruno
A1  -  Prag Hiran A
A1  -  Ruiz-Meana Marisol
A1  -  Szabados Tamara
A1  -  Zuurbier Coert J
A1  -  Ferdinandy Péter
A1  -  Hausenloy Derek J
TI  - IMproving Preclinical Assessment of Cardioprotective Therapies (IMPACT): a small animal acute myocardial infarction randomized-controlled multicenter study on the effect of ischemic preconditioning
AV  - public
EP  - 346
N2  - Although many cardioprotective interventions have been shown to limit infarct size (IS), in preclinical animal studies of acute myocardial ischemia/reperfusion injury (IRI), their clinical translation to patient benefit has been largely disappointing. A major factor is the lack of rigor and reproducibility in the preclinical studies. To address this, we have established the IMproving Preclinical Assessment of Cardioprotective Therapies (IMPACT) small animal multisite acute myocardial infarction (AMI) network, with centralized randomization and blinded core laboratory IS analysis, and have validated the network using ischemic preconditioning (IPC). Eight sites from the COST Innovators Grant (IG16225) network participated in the IMPACT AMI study. Mice and rats were randomly allocated into Sham, Control, or IPC groups. The IRI group underwent 45 min (mice) or 30 min (rats) of left coronary artery occlusion followed by 24 h reperfusion. IPC comprised three cycles of 5 min occlusion/reperfusion before IRI. IS was determined by a blinded core lab. The majority of site showed significant cardioprotection with IPC. In pooled mouse data, IPC (N = 42) reduced IS/AAR by 35% compared to control (N = 48) (30 ą 16% versus 46 ą 13%; p < 0.005), and in rat data, IPC (N = 36) reduced IS/AAR by 29% when compared to control (N = 39) (32 ą 19% versus 45 ą 14%; p < 0.01). The IMPACT multisite mouse and rat AMI networks, with centralized randomization and blinded core IS analysis, were established to improve the reproducibility of cardioprotective interventions in preclinical studies and to facilitate the translation of these therapies for patient benefit.
Y1  - 2025///
SP  - 335
SN  - 0300-8428
UR  - https://doi.org/10.1007/s00395-025-01102-3
ID  - publicatio36293
VL  - 120
JF  - BASIC RESEARCH IN CARDIOLOGY
ER  -