relation: http://publicatio.bibl.u-szeged.hu/36293/ title: IMproving Preclinical Assessment of Cardioprotective Therapies (IMPACT): a small animal acute myocardial infarction randomized-controlled multicenter study on the effect of ischemic preconditioning creator: Hernandez-Resendiz Sauri creator: Vilskersts Reinis creator: Aluja David creator: Andreadou Ioanna creator: Bencsik Péter creator: Dambrova Maija creator: Efentakis Panagiotis creator: Gao Fei creator: Giricz Zoltán creator: Inserte Javier creator: Kelly-Laubscher Roisin creator: Kiss Attila creator: Krieg Thomas creator: Kwak Brenda R creator: Lecour Sandrine creator: Lopaschuk Gary creator: Mączewski Michał creator: Waszkiewicz Michał creator: Oknińska Marta creator: Pagliaro Pasquale creator: Podesser Bruno creator: Prag Hiran A creator: Ruiz-Meana Marisol creator: Szabados Tamara creator: Zuurbier Coert J creator: Ferdinandy Péter creator: Hausenloy Derek J subject: 03.02. Klinikai orvostan description: Although many cardioprotective interventions have been shown to limit infarct size (IS), in preclinical animal studies of acute myocardial ischemia/reperfusion injury (IRI), their clinical translation to patient benefit has been largely disappointing. A major factor is the lack of rigor and reproducibility in the preclinical studies. To address this, we have established the IMproving Preclinical Assessment of Cardioprotective Therapies (IMPACT) small animal multisite acute myocardial infarction (AMI) network, with centralized randomization and blinded core laboratory IS analysis, and have validated the network using ischemic preconditioning (IPC). Eight sites from the COST Innovators Grant (IG16225) network participated in the IMPACT AMI study. Mice and rats were randomly allocated into Sham, Control, or IPC groups. The IRI group underwent 45 min (mice) or 30 min (rats) of left coronary artery occlusion followed by 24 h reperfusion. IPC comprised three cycles of 5 min occlusion/reperfusion before IRI. IS was determined by a blinded core lab. The majority of site showed significant cardioprotection with IPC. In pooled mouse data, IPC (N = 42) reduced IS/AAR by 35% compared to control (N = 48) (30 ± 16% versus 46 ± 13%; p < 0.005), and in rat data, IPC (N = 36) reduced IS/AAR by 29% when compared to control (N = 39) (32 ± 19% versus 45 ± 14%; p < 0.01). The IMPACT multisite mouse and rat AMI networks, with centralized randomization and blinded core IS analysis, were established to improve the reproducibility of cardioprotective interventions in preclinical studies and to facilitate the translation of these therapies for patient benefit. date: 2025 type: Folyóiratcikk type: PeerReviewed format: text identifier: http://publicatio.bibl.u-szeged.hu/36293/7/35909240.pdf format: text identifier: http://publicatio.bibl.u-szeged.hu/36293/1/Hernandez.pdf identifier: Hernandez-Resendiz Sauri; Vilskersts Reinis; Aluja David; Andreadou Ioanna; Bencsik Péter; Dambrova Maija; Efentakis Panagiotis; Gao Fei; Giricz Zoltán; Inserte Javier; Kelly-Laubscher Roisin; Kiss Attila; Krieg Thomas; Kwak Brenda R; Lecour Sandrine; Lopaschuk Gary; Mączewski Michał; Waszkiewicz Michał; Oknińska Marta; Pagliaro Pasquale; Podesser Bruno; Prag Hiran A; Ruiz-Meana Marisol; Szabados Tamara; Zuurbier Coert J; Ferdinandy Péter; Hausenloy Derek J: IMproving Preclinical Assessment of Cardioprotective Therapies (IMPACT): a small animal acute myocardial infarction randomized-controlled multicenter study on the effect of ischemic preconditioning. BASIC RESEARCH IN CARDIOLOGY, 120. pp. 335-346. ISSN 0300-8428 (2025) identifier: doi:10.1007/s00395-025-01102-3 relation: https://doi.org/10.1007/s00395-025-01102-3 relation: 35909240 language: eng relation: info:eu-repo/semantics/altIdentifier/doi/10.1007/s00395-025-01102-3 rights: info:eu-repo/semantics/openAccess