relation: http://publicatio.bibl.u-szeged.hu/35853/ title: Dysbiosis is associated with the behavioral phenotype observed in the triple-hit Wisket rat model of schizophrenia creator: Plesz Szonja Bianka creator: Adlan Leatitia Gabriella creator: Büki Alexandra creator: Makra Nóra creator: Ligeti Balázs creator: Ágg Bence creator: Szabó Dóra creator: Zádori Zoltán S creator: Ferdinandy Péter creator: Horváth Gyöngyi creator: Kékesi Gabriella subject: 03.01. Általános orvostudomány description: Comorbidities between gastrointestinal diseases and psychiatric disorders have been widely reported, with the gut-brain axis implicated as a potential biological basis. Thus, dysbiosis may play an important role in the etiology of schizophrenia, which is barely detected. Triple-hit Wisket model rats exhibit various schizophrenia-like behavioral phenotypes. The present study aimed to compare the diversity and abundance of gut microbiota in Wisket model and control rats; furthermore, to correlate the microbial taxonomic profiles to indices of behavioral change. Tail-flick and Ambitus tests were used to assess acute heat pain sensitivity, and record exploration and locomotor activity along with motivation in young adult, control and Wisket model rats. Fecal microbiota composition was profiled by deep sequencing of bacterial 16S rRNA, and it was correlated to behavioral phenotype. Wisket rats exhibited significantly decreased pain sensitivity, lower locomotor activity and exploration, and impaired motivation compared with controls. No significant differences were observed in bacterial alpha diversity between the groups; however, clear differences in community structure were observed. Wisket rats showed decreases in several genera of Firmicutes and Saccharimonas, and increases in Bacteriodetes and Helicobacter phyla compared with controls. Correlation analysis revealed significant associations between the microbiota profile and the behavioral phenotype. This is the first demonstration that fecal microbiota composition is markedly altered in a triple-hit schizophrenia rat model, suggesting the contribution of the microbiota-gut-brain axis in the development of the schizophrenia-like behavioral phenotype. Thus targeting the gut microbiota may be a novel approach to treat such impairments. date: 2025 type: Folyóiratcikk type: PeerReviewed format: text identifier: http://publicatio.bibl.u-szeged.hu/35853/1/35726378.pdf identifier: Plesz Szonja Bianka; Adlan Leatitia Gabriella; Büki Alexandra; Makra Nóra; Ligeti Balázs; Ágg Bence; Szabó Dóra; Zádori Zoltán S; Ferdinandy Péter; Horváth Gyöngyi; Kékesi Gabriella: Dysbiosis is associated with the behavioral phenotype observed in the triple-hit Wisket rat model of schizophrenia. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, 137. ISSN 0278-5846 (2025) identifier: doi:10.1016/j.pnpbp.2025.111276 relation: https://doi.org/10.1016/j.pnpbp.2025.111276 relation: 35726378 language: eng relation: info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pnpbp.2025.111276 rights: info:eu-repo/semantics/openAccess