TY - JOUR ID - publicatio35541 TI - Prospective validation of ORACLE, a clonal expression biomarker associated with survival of patients with lung adenocarcinoma SP - 86 A1 - Biswas Dhruva A1 - Liu Yun-Hsin A1 - Herrero Javier A1 - Snell Daniel M. A1 - O?Neill Olga A1 - Leonce Daniel A1 - Mattsson Johanna A1 - Lindberg Amanda A1 - Micke Patrick A1 - Moldvay Judit A1 - Megyesfalvi Zsolt A1 - Döme Balazs A1 - Fillinger János A1 - Nicod Jerome A1 - Downward Julian A1 - Kollaboráiós szervezet: TRACERx Consortium A1 - et al. Y1 - 2025/// UR - https://doi.org/10.1038/s43018-024-00883-1 N2 - Human tumors are diverse in their natural history and response to treatment, which in part results from genetic and transcriptomic heterogeneity. In clinical practice, single-site needle biopsies are used to sample this diversity, but cancer biomarkers may be confounded by spatiogenomic heterogeneity within individual tumors. Here we investigate clonally expressed genes as a solution to the sampling bias problem by analyzing multiregion whole-exome and RNA sequencing data for 450 tumor regions from 184 patients with lung adenocarcinoma in the TRACERx study. We prospectively validate the survival association of a clonal expression biomarker, Outcome Risk Associated Clonal Lung Expression (ORACLE), in combination with clinicopathological risk factors, and in stage I disease. We expand our mechanistic understanding, discovering that clonal transcriptional signals are detectable before tissue invasion, act as a molecular fingerprint for lethal metastatic clones and predict chemotherapy sensitivity. Lastly, we find that ORACLE summarizes the prognostic information encoded by genetic evolutionary measures, including chromosomal instability, as a concise 23-transcript assay. VL - 6 N1 - Online ahead of print. Published online: 09 January 2025 EP - 101 IS - 1 SN - 2662-1347 AV - restricted JF - NATURE CANCER ER -