TY  - JOUR
TI  - Genotypic Characterisation and Antimicrobial Resistance of Extended-Spectrum ?-Lactamase-Producing Escherichia coli in Humans, Animals, and the Environment from Lusaka, Zambia: Public Health Implications and One Health Surveillance
IS  - 10
JF  - ANTIBIOTICS
UR  - https://doi.org/10.3390/antibiotics13100951
N2  - Background: Extended-spectrum ?-lactamases (ESBL) in Escherichia coli are a serious concern due to their role in developing multidrug resistance (MDR) and difficult-to-treat infections. Objective: This study aimed to identify ESBL-carrying E. coli strains from both clinical and environmental sources in Lusaka District, Zambia. Methods: This cross-sectional study included 58 ESBL-producing E. coli strains from hospital inpatients, outpatients, and non-hospital environments. Antimicrobial susceptibility was assessed using the Kirby?Bauer disk diffusion method and the VITEK® 2 Compact System, while genotypic analyses utilised the Illumina NextSeq 2000 sequencing platform. Results: Among the strains isolated strains, phylogroup B2 was the most common, with resistant MLST sequence types including ST131, ST167, ST156, and ST69. ESBL genes such as blaTEM-1B, blaCTX-M,blaOXA-1, blaNDM-5, and blaCMY were identified, with ST131 and ST410 being the most common. ST131 exhibited a high prevalence of blaCTX-M-15 and resistance to fluoroquinolones. Clinical and environmental isolates carried blaNDM-5 (3.4%), with clinical isolates showing a higher risk of carbapenemase resistance genes and the frequent occurrence of blaCTX-M and blaTEM variants, especially blaCTX-M-15 in ST131. Conclusions: This study underscores the public health risks of blaCTX-M-15- and blaNDM-5-carrying E. coli. The strengthening antimicrobial stewardship programmes and the continuous surveillance of AMR in clinical and environmental settings are recommended to mitigate the spread of resistant pathogens.
Y1  - 2024///
A1  -  Kasanga Maisa
A1  -  Gajdács Márió
A1  -  Muleya Walter
A1  -  Ikhimiukor Odion O.
A1  -  Mudenda Steward
A1  -  Kasanga Maika
A1  -  Chizimu Joseph
A1  -  Shempela Doreen Mainza
A1  -  Solochi Benjamin Bisesa
A1  -  Mwikisa Mark John
A1  -  Yamba Kaunda
A1  -  Andam Cheryl P.
A1  -  Chanda Raphael
A1  -  Chanda Duncan
A1  -  Kwenda Geoffrey
AV  - public
ID  - publicatio35059
SN  - 2079-6382
EP  - 17
VL  - 13
ER  -