%0 Journal Article
%@ 2079-6382
%A  Kasanga Maisa
%A  Gajdács Márió
%A  Muleya Walter
%A  Ikhimiukor Odion O.
%A  Mudenda Steward
%A  Kasanga Maika
%A  Chizimu Joseph
%A  Shempela Doreen Mainza
%A  Solochi Benjamin Bisesa
%A  Mwikisa Mark John
%A  Yamba Kaunda
%A  Andam Cheryl P.
%A  Chanda Raphael
%A  Chanda Duncan
%A  Kwenda Geoffrey
%A Orálbiológiai és Kísérletes Fogorvostudományi Tanszék SZTE / FOK OKFT [2012-],
%D 2024
%F publicatio:35059
%J ANTIBIOTICS
%N 10
%T Genotypic Characterisation and Antimicrobial Resistance of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Humans, Animals, and the Environment from Lusaka, Zambia: Public Health Implications and One Health Surveillance
%U http://publicatio.bibl.u-szeged.hu/35059/
%V 13
%X Background: Extended-spectrum β-lactamases (ESBL) in Escherichia coli are a serious concern due to their role in developing multidrug resistance (MDR) and difficult-to-treat infections. Objective: This study aimed to identify ESBL-carrying E. coli strains from both clinical and environmental sources in Lusaka District, Zambia. Methods: This cross-sectional study included 58 ESBL-producing E. coli strains from hospital inpatients, outpatients, and non-hospital environments. Antimicrobial susceptibility was assessed using the Kirby–Bauer disk diffusion method and the VITEK® 2 Compact System, while genotypic analyses utilised the Illumina NextSeq 2000 sequencing platform. Results: Among the strains isolated strains, phylogroup B2 was the most common, with resistant MLST sequence types including ST131, ST167, ST156, and ST69. ESBL genes such as blaTEM-1B, blaCTX-M,blaOXA-1, blaNDM-5, and blaCMY were identified, with ST131 and ST410 being the most common. ST131 exhibited a high prevalence of blaCTX-M-15 and resistance to fluoroquinolones. Clinical and environmental isolates carried blaNDM-5 (3.4%), with clinical isolates showing a higher risk of carbapenemase resistance genes and the frequent occurrence of blaCTX-M and blaTEM variants, especially blaCTX-M-15 in ST131. Conclusions: This study underscores the public health risks of blaCTX-M-15- and blaNDM-5-carrying E. coli. The strengthening antimicrobial stewardship programmes and the continuous surveillance of AMR in clinical and environmental settings are recommended to mitigate the spread of resistant pathogens.