relation: http://publicatio.bibl.u-szeged.hu/35059/
title: Genotypic Characterisation and Antimicrobial Resistance of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Humans, Animals, and the Environment from Lusaka, Zambia: Public Health Implications and One Health Surveillance
creator:  Kasanga Maisa
creator:  Gajdács Márió
creator:  Muleya Walter
creator:  Ikhimiukor Odion O.
creator:  Mudenda Steward
creator:  Kasanga Maika
creator:  Chizimu Joseph
creator:  Shempela Doreen Mainza
creator:  Solochi Benjamin Bisesa
creator:  Mwikisa Mark John
creator:  Yamba Kaunda
creator:  Andam Cheryl P.
creator:  Chanda Raphael
creator:  Chanda Duncan
creator:  Kwenda Geoffrey
subject: 03.03. Egészségtudományok
description: Background: Extended-spectrum β-lactamases (ESBL) in Escherichia coli are a serious concern due to their role in developing multidrug resistance (MDR) and difficult-to-treat infections. Objective: This study aimed to identify ESBL-carrying E. coli strains from both clinical and environmental sources in Lusaka District, Zambia. Methods: This cross-sectional study included 58 ESBL-producing E. coli strains from hospital inpatients, outpatients, and non-hospital environments. Antimicrobial susceptibility was assessed using the Kirby–Bauer disk diffusion method and the VITEK® 2 Compact System, while genotypic analyses utilised the Illumina NextSeq 2000 sequencing platform. Results: Among the strains isolated strains, phylogroup B2 was the most common, with resistant MLST sequence types including ST131, ST167, ST156, and ST69. ESBL genes such as blaTEM-1B, blaCTX-M,blaOXA-1, blaNDM-5, and blaCMY were identified, with ST131 and ST410 being the most common. ST131 exhibited a high prevalence of blaCTX-M-15 and resistance to fluoroquinolones. Clinical and environmental isolates carried blaNDM-5 (3.4%), with clinical isolates showing a higher risk of carbapenemase resistance genes and the frequent occurrence of blaCTX-M and blaTEM variants, especially blaCTX-M-15 in ST131. Conclusions: This study underscores the public health risks of blaCTX-M-15- and blaNDM-5-carrying E. coli. The strengthening antimicrobial stewardship programmes and the continuous surveillance of AMR in clinical and environmental settings are recommended to mitigate the spread of resistant pathogens.
date: 2024
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/35059/1/antibiotics-13-00951-v2.pdf
identifier:     Kasanga Maisa;  Gajdács Márió;  Muleya Walter;  Ikhimiukor Odion O.;  Mudenda Steward;  Kasanga Maika;  Chizimu Joseph;  Shempela Doreen Mainza;  Solochi Benjamin Bisesa;  Mwikisa Mark John;  Yamba Kaunda;  Andam Cheryl P.;  Chanda Raphael;  Chanda Duncan;  Kwenda Geoffrey: Genotypic Characterisation and Antimicrobial Resistance of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Humans, Animals, and the Environment from Lusaka, Zambia: Public Health Implications and One Health Surveillance.   ANTIBIOTICS, 13 (10).   ISSN 2079-6382 (2024)     
identifier: doi:10.3390/antibiotics13100951
relation: https://doi.org/10.3390/antibiotics13100951
relation: 35501300
language: eng
relation: info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics13100951
rights: info:eu-repo/semantics/openAccess