TY  - JOUR
AV  - public
ID  - publicatio34360
Y1  - 2006///
A1  -  Kiss Tamás
A1  -  Jakusch Tamás
A1  -  Bouhsina Saâd
A1  -  Sakurai Hiromu
A1  -  Enyedy Éva Anna
SN  - 1434-1948
EP  - 3613
VL  - 2006
JF  - EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
TI  - Binding Constant of VIVO to Transferrin
IS  - 18
N2  - The interactions of bis-(VO)-O-IV-1,2-dimethyl-3-hydroxy-4(1H)pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed, and the reaction was used to determine the binding constant of (VO)-O-IV to apoTF. A value of log K = 14.3 +/- 0.6 was obtained for the binding of the first (VO)-O-IV to apoTF In order to confirm the validity of the binding constant, UV spectroscopy was used to monitor the displacement reaction between (VO)-O-IV-apoTF and nitrilotriacetic acid (NTA). The difference between the binding constants obtained by the two different methods (ca. one log unit) was explained in terms of the possible ternary complex formation between (VO)-O-IV-apoTF and the displacing ligand. The binding constants obtained exhibit a reasonably good agreement with the linear free energy relationship (LFER) estimation.
SP  - 3607
UR  - https://doi.org/10.1002/ejic.200600314
ER  -