relation: http://publicatio.bibl.u-szeged.hu/34360/
title: Binding Constant of VIVO to Transferrin
creator:  Kiss Tamás
creator:  Jakusch Tamás
creator:  Bouhsina Saâd
creator:  Sakurai Hiromu
creator:  Enyedy Éva Anna
subject: 01.04. Kémiai tudományok
description: The interactions of bis-(VO)-O-IV-1,2-dimethyl-3-hydroxy-4(1H)pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed, and the reaction was used to determine the binding constant of (VO)-O-IV to apoTF. A value of log K = 14.3 +/- 0.6 was obtained for the binding of the first (VO)-O-IV to apoTF In order to confirm the validity of the binding constant, UV spectroscopy was used to monitor the displacement reaction between (VO)-O-IV-apoTF and nitrilotriacetic acid (NTA). The difference between the binding constants obtained by the two different methods (ca. one log unit) was explained in terms of the possible ternary complex formation between (VO)-O-IV-apoTF and the displacing ligand. The binding constants obtained exhibit a reasonably good agreement with the linear free energy relationship (LFER) estimation.
date: 2006
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/34360/1/Eur_J_Inorg_Chem_2006_3607.pdf
identifier:     Kiss Tamás;  Jakusch Tamás;  Bouhsina Saâd;  Sakurai Hiromu;  Enyedy Éva Anna: Binding Constant of VIVO to Transferrin.   EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2006 (18).  pp. 3607-3613.  ISSN 1434-1948 (2006)     
identifier: doi:10.1002/ejic.200600314
relation: https://doi.org/10.1002/ejic.200600314
relation: 1087333
language: eng
relation: info:eu-repo/semantics/altIdentifier/doi/10.1002/ejic.200600314
rights: info:eu-repo/semantics/openAccess