%N 9
%D 1992
%X The arachidonic acid metabolite 12-hydroxyeicosatetraenoic acid
(12-HETE) is the main eicosanoid formed by epidermal cells and is
assumed to play an important role in skin physiology and
pathophysiology. Our aim was to find out whether epidermal Langerhans
cells possess specific receptors for 12-HETE which would mediate the
effects of this eicosanoid in their skin microenvironment. By
radioligand binding studies on isolated human Langerhans cells, we
could identify specific binding sites for 12(S)-HETE. The analysis of
binding data revealed a single class of binding sites with a K(d) of
3.32 +/- 0.45 nm and a B(max) of 691 000 +/- 58 000 receptors per
cell.The binding was saturable, readily reversible, and specific for
12(S)-HETE. The receptor is likely to mediate the potent chemotactic
response of human Langerhans cells towards 12(S)-HETE, which we
previously described. Our results strongly suggest that extremely low
concentrations of 12-HETE which is formed by epidermal keratinocytes
may dramatically influence the biology of Langerhans cells by
receptor-mediated effects.
%P 2469-2472
%I mi
%R MTMT:1125089 10.1002/eji.1830220944
%A  Arenberger P
%A  KemĂŠny Lajos
%A  Rupec R
%A  Bieber T
%A  Ruzicka T
%L publicatio32202
%T Langerhans Cells Of The Human Skin Possess High-affinity 12(s)-hydroxyeicosa Tetraenoic Acid Receptors
%V 22
%J EUROPEAN JOURNAL OF IMMUNOLOGY