%0 Journal Article
%@ 0148-3919
%A  Szókán Gyula
%A  Hadfi S
%A  Krizsán Krisztina
%A  Liembeck A
%A  Krecz I
%A  Almás M
%A  Somlai Csaba
%A Orvosi Vegytani Intézet SZTE / SZAOK OVI [2000-],
%D 1994
%F publicatio:31995
%J JOURNAL OF LIQUID CHROMATOGRAPHY
%N 13
%P 2759-2775
%T Hplc determination of enantiomeric purity of protected amino acid derivatives used in peptide synthesis
%U http://publicatio.bibl.u-szeged.hu/31995/
%V 17
%X An improved RP-HPLC method on ODS-Hypersil column with precolumn derivatization with Marfey's reagent were used to monitor racemization in N-, C- and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic samples were obtained by partial deprotection of different starting materials. In a simple two-step procedure (deprotection and derivatization) the compounds of amino acids formed stable diastereomeric derivatives having facile resolutions.
%Z Z9: 13 WC: Biochemical Research Methods; Chemistry, Analytical