%P 309-311 %T Chemotaxis of freshly separated and cultured human keratinocytes %V 19 %D 1994 %L publicatio31958 %X It is generally accepted that keratinocyte migration plays a critical role in the process of wound healing. A study was therefore made of the migratory response of freshly separated and cultured human keratinocytes to factors with chemotactic properties for a variety of cells. Interleukin-1alpha (IL-1alpha), interferon-gamma (IFN-gamma), interleukin-8 (IL-8), and tumour necrosis factor alpha (TNF-alpha) were tested for their chemotactic effectiveness in a modified Boyden chamber assay. IFN-gamma, IL-1alpha and IL-8 were demonstrated to serve as chemoattractants for freshly separated keratinocytes. For cultured cells, however, only IFN-gamma was found to display chemotactic properties. The findings demonstrate that there is significant difference between the chemotactic behaviour of freshly separated and cultured cells. %N 4 %R MTMT:105842 10.1111/j.1365-2230.1994.tb01201.x %A Gyulai Rolland PĂŠter %A Hunyadi JĂĄnos %A Kenderessy SzabĂł Anna %A KemĂŠny Lajos %A Dobozy Attila %I szgyaoe %J CLINICAL AND EXPERIMENTAL DERMATOLOGY