TY - JOUR VL - 19 A1 - Gyulai Rolland Péter A1 - Hunyadi János A1 - Kenderessy Szabó Anna A1 - Kemény Lajos A1 - Dobozy Attila IS - 4 ID - publicatio31958 SN - 0307-6938 UR - https://doi.org/10.1111/j.1365-2230.1994.tb01201.x SP - 309 EP - 311 JF - CLINICAL AND EXPERIMENTAL DERMATOLOGY N2 - It is generally accepted that keratinocyte migration plays a critical role in the process of wound healing. A study was therefore made of the migratory response of freshly separated and cultured human keratinocytes to factors with chemotactic properties for a variety of cells. Interleukin-1alpha (IL-1alpha), interferon-gamma (IFN-gamma), interleukin-8 (IL-8), and tumour necrosis factor alpha (TNF-alpha) were tested for their chemotactic effectiveness in a modified Boyden chamber assay. IFN-gamma, IL-1alpha and IL-8 were demonstrated to serve as chemoattractants for freshly separated keratinocytes. For cultured cells, however, only IFN-gamma was found to display chemotactic properties. The findings demonstrate that there is significant difference between the chemotactic behaviour of freshly separated and cultured cells. Y1 - 1994/// AV - restricted TI - Chemotaxis of freshly separated and cultured human keratinocytes ER -