%0 Journal Article
%@ 0307-6938
%A  Gyulai Rolland Péter
%A  Hunyadi János
%A  Kenderessy Szabó Anna
%A  Kemény Lajos
%A  Dobozy Attila
%A Bőrgyógyászati Tanszék DE / ÁOK BorgyogyT [2014-2021],
%A Bőrgyógyászati és Allergológiai Klinika SZTE / SZAOK BAK [2002-],
%D 1994
%F publicatio:31958
%J CLINICAL AND EXPERIMENTAL DERMATOLOGY
%N 4
%P 309-311
%T Chemotaxis of freshly separated and cultured human keratinocytes
%U http://publicatio.bibl.u-szeged.hu/31958/
%V 19
%X It is generally accepted that keratinocyte migration plays a critical role in the process of wound healing. A study was therefore made of the migratory response of freshly separated and cultured human keratinocytes to factors with chemotactic properties for a variety of cells. Interleukin-1alpha (IL-1alpha), interferon-gamma (IFN-gamma), interleukin-8 (IL-8), and tumour necrosis factor alpha (TNF-alpha) were tested for their chemotactic effectiveness in a modified Boyden chamber assay. IFN-gamma, IL-1alpha and IL-8 were demonstrated to serve as chemoattractants for freshly separated keratinocytes. For cultured cells, however, only IFN-gamma was found to display chemotactic properties. The findings demonstrate that there is significant difference between the chemotactic behaviour of freshly separated and cultured cells.