relation: http://publicatio.bibl.u-szeged.hu/29661/
title: Translational Capacity of a Cell Is Determined during Transcription Elongation via the Ccr4-Not Complex
creator:  Gupta Ishaan
creator:  Villanyi Zoltan
creator:  Kassem Sari
creator:  Hughes Christopher
creator:  Panasenko Olesya O
creator:  Steinmetz Lars M
creator:  Collart Martine A
subject: 01.06. Biológiai tudományok
description: The current understanding of gene expression considers transcription and translation to be independent processes. Challenging this notion, we found that translation efficiency is determined during transcription elongation through the imprinting of mRNAs with Not1, the central scaffold of the Ccr4-Not complex. We determined that another subunit of the complex, Not5, defines Not1 binding to specific mRNAs, particularly those produced from ribosomal protein genes. This imprinting mechanism specifically regulates ribosomal protein gene expression, which in turn determines the translational capacity of cells. We validate our model by SILAC and polysome profiling experiments. As a proof of concept, we demonstrate that enhanced translation compensates for transcriptional elongation stress. Taken together, our data indicate that in addition to defining mRNA stability, components of the Ccr4-Not imprinting complex regulate RNA translatability, thus ensuring global gene expression homeostasis.
date: 2016
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/29661/3/26043441.pdf
identifier:     Gupta Ishaan;  Villanyi Zoltan;  Kassem Sari;  Hughes Christopher;  Panasenko Olesya O;  Steinmetz Lars M;  Collart Martine A: Translational Capacity of a Cell Is Determined during Transcription Elongation via the Ccr4-Not Complex.   CELL REPORTS, 15 (8).  pp. 1782-1794.  ISSN 2211-1247 (2016)     
identifier: doi:10.1016/j.celrep.2016.04.055
relation: https://doi.org/10.1016/j.celrep.2016.04.055
relation: 26043441
language: eng