relation: http://publicatio.bibl.u-szeged.hu/27182/
title: Effect of liposomal formulation of ascorbic acid on corneal permeability
creator:  Csorba Anita
creator:  Katona Gábor
creator:  Budai-Szűcs Mária
creator:  Balogh Weiser Diána
creator:  Fadda Anna Maria
creator:  Carla Caddeo
creator:  Takács Ágnes Ildikó
creator:  Mátyus Péter
creator:  Balogh György Tibor
creator:  Nagy Zoltán Zsolt
subject: 03.01. Általános orvostudomány
subject: 03.02. Klinikai orvostan
description: Ascorbic acid (AA) has a pivotal role in corneal wound healing via stimulating the biosynthesis of highly organized extracellular matrix components, but its rapid degradation and low corneal permeability limits its therapeutic effects. In this paper, we present the pharmacokinetic properties of a liposomal-based formulation of AA in terms of corneal permeation. Chemical stability, shelf-life, and drug release rate of lyophilized liposome (AA-LLipo) formulation was determined in comparison to free-form of AA solution using high-performance liquid chromatography (HPLC) and rapid equilibrium dialysis. In vitro transcorneal permeability was studied using a parallel artificial membrane permeability assay (PAMPA). Ex vivo permeation was examined on AA-LLipo-treated porcine cornea by determining the AA content on the ocular surface, in the cornea as well as in the aqueous humor using HPLC, and by Raman-mapping visualizing the AA-distribution. Our results showed that the liposomal formulation improved the chemical stability of AA, while drug release was observed with the same kinetic efficiency as from the free-form of AA solution. Both corneal-PAMPA and porcine corneal permeability studies showed that AA-LLipo markedly improved the corneal absorption kinetics of AA, thus, increasing the AA content in the cornea and aqueous humor. AA-LLipo formulation could potentially increase the bioavailability of AA in corneal tissues.
date: 2023
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/27182/1/Csorba_s41598-023-29290-9.pdf
identifier:     Csorba Anita;  Katona Gábor;  Budai-Szűcs Mária;  Balogh Weiser Diána;  Fadda Anna Maria;  Carla Caddeo;  Takács Ágnes Ildikó;  Mátyus Péter;  Balogh György Tibor;  Nagy Zoltán Zsolt: Effect of liposomal formulation of ascorbic acid on corneal permeability.   SCIENTIFIC REPORTS, 13 (1).   ISSN 2045-2322 (2023)     
identifier: doi:10.1038/s41598-023-29290-9
relation: https://doi.org/10.1038/s41598-023-29290-9
relation: 33675955
language: eng