relation: http://publicatio.bibl.u-szeged.hu/26467/
title: Lineage Reconstruction of In Vitro Identified Antigen-Specific Autoreactive B Cells from Adaptive Immune Receptor Repertoires
creator:  Blazsó Péter
creator:  Csomos Krisztian
creator:  Tipton Christopher M.
creator:  Ujhazi Boglarka
creator:  Walter Jolan E.
subject: 01.06. Biológiai tudományok
subject: 03.01. Általános orvostudomány
description: The emergence, survival, growth and maintenance of autoreactive (AR) B-cell clones, the hallmark of humoral autoimmunity, leave their footprints in B-cell receptor repertoires. Collecting IgH sequences related to polyreactive (PR) ones from adaptive immune receptor repertoire (AIRR) datasets make the reconstruction and analysis of PR/AR B-cell lineages possible. We developed a computational approach, named ImmChainTracer, to extract members and to visualize clonal relationships of such B-cell lineages. Our approach was successfully applied on the IgH repertoires of patients suffering from monogenic hypomorphic RAG1 and 2 deficiency (pRD) or polygenic systemic lupus erythematosus (SLE) autoimmune diseases to identify relatives of AR IgH sequences and to track their fate in AIRRs. Signs of clonal expansion, affinity maturation and class-switching events in PR/AR and non-PR/AR B-cell lineages were revealed. An extension of our method towards B-cell expansion caused by any trigger (e.g., infection, vaccination or antibody development) may provide deeper insight into antigen specific B-lymphogenesis.
date: 2023
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/26467/1/ijms-24-00225.pdf
identifier:     Blazsó Péter;  Csomos Krisztian;  Tipton Christopher M.;  Ujhazi Boglarka;  Walter Jolan E.: Lineage Reconstruction of In Vitro Identified Antigen-Specific Autoreactive B Cells from Adaptive Immune Receptor Repertoires.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (1).   ISSN 1661-6596 (2023)     
identifier: doi:10.3390/ijms24010225
relation: https://doi.org/10.3390/ijms24010225
relation: 33552340
language: eng
relation: info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms24010225
rights: info:eu-repo/semantics/openAccess