%0 Journal Article
%@ 1664-302X
%A  Gomez-Molero Emilia
%A  Willis Jesse R.
%A  Dudakova Anna
%A  Carrete Laia
%A  Weig Michael
%A  Groß Uwe
%A  Gácser Attila
%A  Gabaldón Toni
%A  Bader Oliver
%A Mikrobiológiai Tanszék SZTE / TTIK / BI MBT [2016-],
%A MTA-SZTE Lendület Mikobiom Kutatócsoport SZTE / TTIK / BI / MBT [2018-],
%D 2020
%F publicatio:22014
%J FRONTIERS IN MICROBIOLOGY
%P Terjedelem: 10-Azonosító: 1994
%T Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages
%U http://publicatio.bibl.u-szeged.hu/22014/
%V 11
%X The human pathogenic yeastCandida parapsilosishas gained significant importance over the past decades as one of the principal causes of fungal bloodstream infections. Isolates ofC. parapsilosisare known to be able to switch between several different colony morphologies in vitro, which are correlated with different cell shapes, altered cell surface properties, and thus different capacities to form biofilms on indwelling medical devices. In a set of six clinical specimens from a single surgery patient yielding stable smooth- as well as crepe-morphology isolates, we investigated the differences between five of them on a phenotypic and genomic level. In contrast to the initial assumption that they were switched forms of a clonal strain, karyotyping and genome sequencing showed that the patient was colonized by at least three distinct linages. Statistical analysis placed these groups distantly across the population ofC. parapsilosis. Interestingly the single blood culture isolate was of smooth morphology and matched with an isolate from the patient's nose of similar morphology. Strong variation between the isolates was seen in adhesin-encoding genes, where repeat regions showed significant variation in length and repeat-numbers, most strikingly inHWP1of the smooth isolates. Although no differences in drug susceptibility were evident, the high phylogenetic distance separating the individual strains highlights the need for testing of multiple colonies in routine practice. The absence of biofilm formation in the blood stream isolate indicates a lack of respective adhesins in the cell wall, in turn pointing toward lack of adhesion as a positively contributing factor for dissemination.