relation: http://publicatio.bibl.u-szeged.hu/19833/
title: In silico prediction of active site and in vitro DNase and RNase activities of Helicoverpa-inducible pathogenesis related-4 protein from Cicer arietinum
creator:  Singh Archana
creator:  Jain Deepti
creator:  Tyagi Chetna
creator:  Singh Sujata
creator:  Kumar Sumit
creator:  Singh Indrakant Kumar
description: Plants are endowed with an innate immune system, which enables them to protect themselves from pest and pathogen. The participation of pathogenesis-related (PR) proteins is one of the most crucial events of inducible plant defense response. Herein, we report the characterization of CaHaPR-4, a Helicoverpa-inducible class II PR-4 protein from chickpea. Bioinformatic analysis of CaHaPR-4 protein indicated the presence of a signal peptide, barwin domain but it lacks the chitin-binding site/hevein domain. The recombinant CaHaPR-4 is bestowed with RNase and bivalent ion-dependent DNase activity. Further, the RNA and DNA binding sites were identified and confirmed by analyzing interactions between mutated CaHaPR-4 with the altered active site and ribonuclease inhibitor, 5'ADP and DNase inhibitor, 2 nitro 5 thiocyanobenzoic acid (NTCB) using 3D modeling and docking studies. Moreover, CaHaPR-4 shows antifungal activity as well as growth inhibiting properties against neonatal podborer larvae. To the best of our knowledge, this is the first report of a PR-4 showing RNase, DNase, antifungal and most importantly insect growth inhibiting properties against Helicoverpa armigera simultaneously. (C) 2018 Elsevier B.V. All rights reserved.
date: 2018
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/19833/1/1-s2.0-S0141813018300333-main.pdf
identifier:     Singh Archana;  Jain Deepti;  Tyagi Chetna;  Singh Sujata;  Kumar Sumit;  Singh Indrakant Kumar: In silico prediction of active site and in vitro DNase and RNase activities of Helicoverpa-inducible pathogenesis related-4 protein from Cicer arietinum.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 113.  pp. 869-880.  ISSN 0141-8130 (2018)     
identifier: doi:10.1016/j.ijbiomac.2018.03.027
relation: http://doi.org/10.1016/j.ijbiomac.2018.03.027
relation: 30507997
language: eng