relation: http://publicatio.bibl.u-szeged.hu/18314/
title: Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells is enhanced by an aragonite scaffold
creator:  Matta Csaba
creator:  Somogyi Csilla
creator:  Kon Elizaveta
creator:  Robinson Dror
creator:  Neufeld Tova
creator:  Berta Ágnes
creator:  Altschuler Nir
creator:  Hangody László
creator:  Veréb Zoltán
creator:  Zákány Róza
description: Bone graft substitutes and bone void fillers are predominantly used to treat bone defects and bone fusion in orthopaedic surgery. Some aragonite-based scaffolds of coralline exoskeleton origin exhibit osteoconductive properties and are described as useful bone repair scaffolds. The purpose of this study was to evaluate the in vitro osteogenic potential of the bone phase of a novel aragonite-based bi-phasic osteochondral scaffold (Agili-C™, CartiHeal Ltd.) using adult human bone marrow-derived mesenchymal stem cells (MSCs). Analyses were performed at several time intervals: 3, 7, 14, 21, 28 and 42 days post-seeding. Osteogenic differentiation was assessed by morphological characterisation using light microscopy after Alizarin red and von Kossa staining, and scanning electron microscopy. The transcript levels of alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), bone gamma-carboxyglutamate (BGLAP), osteonectin (SPARC) and osteopontin (SPP1) were determined by quantitative PCR. Proliferation was assessed by a thymidine incorporation assay and proliferating cell nuclear antigen (PCNA) immunocytochemistry. Our results demonstrate that the bone phase of the bi-phasic aragonite-based scaffold supports osteogenic differentiation and enhanced proliferation of bone marrow-derived MSCs at both the molecular and histological levels. The scaffold was colonized by differentiating MSCs, suggesting its suitability for incorporation into bone voids to accelerate bone healing, remodelling and regeneration. The mechanism of osteogenic differentiation involves scaffold surface modification with de novo production of calcium phosphate deposits, as revealed by energy dispersive spectroscopy (EDS) analyses. This novel coral-based scaffold may promote the rapid formation of high quality bone during the repair of osteochondral lesions.
date: 2019
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/18314/1/1-s2.0-S0301468119300040-main1.pdf
identifier:     Matta Csaba;  Somogyi Csilla;  Kon Elizaveta;  Robinson Dror;  Neufeld Tova;  Berta Ágnes;  Altschuler Nir;  Hangody László;  Veréb Zoltán;  Zákány Róza: Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells is enhanced by an aragonite scaffold.   DIFFERENTIATION, 107.  pp. 24-34.  ISSN 0301-4681 (2019)     
identifier: doi:10.1016/j.diff.2019.05.002
relation: http://doi.org/10.1016/j.diff.2019.05.002
relation: 30686163
language: eng