%0 Journal Article
%@ 0362-4803
%A  Schäfer Balázs
%A  Orbán Erika
%A  Kele Zoltán
%A  Tömböly Csaba
%A MTA-ELTE Peptidkémiai Kutatócsoport ELTE / TTK / KI [2007-],
%A MTA Szegedi Biológiai Kutatóközpont [1971-],
%A Biokémiai Intézet MTA SZBK BKI [1971-],
%A Orvosi Vegytani Intézet SZTE / ÁOK OVI [2000-],
%D 2015
%F publicatio:16996
%J JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
%N 1
%P 7-13
%T Tritium labelling of a cholesterol amphiphile designed for cell membrane anchoring of proteins.
%U http://publicatio.bibl.u-szeged.hu/16996/
%V 58
%X Cell membrane association of proteins can be achieved by the addition of lipid moieties to the polypeptide chain, and such lipid-modified proteins have important biological functions. A class of cell surface proteins contains a complex glycosylphosphatidylinositol (GPI) glycolipid at the C-terminus, and they are accumulated in cholesterol-rich membrane microdomains, that is, lipid rafts. Semisynthetic lipoproteins prepared from recombinant proteins and designed lipids are valuable probes and model systems of the membrane-associated proteins. Because GPI-anchored proteins can be reinserted into the cell membrane with the retention of the biological function, they are appropriate candidates for preparing models via reduction of the structural complexity. A synthetic headgroup was added to the 3beta-hydroxyl group of cholesterol, an essential lipid component of rafts, and the resulting cholesterol derivative was used as a simplified GPI mimetic. In order to quantitate the membrane integrated GPI mimetic after the exogenous addition to live cells, a tritium labelled cholesterol anchor was prepared. The radioactive label was introduced into the headgroup, and the radiolabelled GPI mimetic anchor was obtained with a specific activity of 1.37 TBq/mmol. The headgroup labelled cholesterol derivative was applied to demonstrate the sensitive detection of the cell membrane association of the anchor under in vivo conditions.
%Z Admin megjegyzés-24720161 #JournalID1# Name: J LABELLED COMPD RAD ISSN: 0362-4803 #JournalID2#