relation: http://publicatio.bibl.u-szeged.hu/14980/
title: Flavonol 7-O-Glucoside Herbacitrin Inhibits HIV-1 Replication through Simultaneous Integrase and Reverse Transcriptase Inhibition
creator:  Áy Eva
creator:  Hunyadi Attila
creator:  Mezei Mária
creator:  Minárovits János
creator:  Hohmann Judit
description: Here we report the evaluation of the antiretroviral effect of two flavonoid 7-O-glucosides, herbacitrin (1) and gossypitrin (2), together with quercetin (3), a well-studied flavonol. Antiviral activity of the flavonoids was assessed by analyzing HIV-1 p24 core protein levels in the supernatants of HIV-1 infected MT-4 and MT-2 cell cultures. The compounds showed mild to weak cytotoxic activities on the host cells; herbacitrin was the strongest in this regard (CC50=27.8 and 63.64 μM on MT-4 and MT-2 cells, respectively). In nontoxic concentrations, herbacitrin and quercetin reduced HIV-1 replication, whereas gossypitrin was ineffective. Herbacitrin was found to inhibit reverse transcriptase at 21.5 μM, while it was a more potent integrase inhibitor already active at 2.15 μM. Therefore, our observations suggest that herbacitrin exerts antiretroviral activity through simultaneously acting on these two targets of HIV-1 and that integrase inhibition might play a major role in this activity.
date: 2019
type: Folyóiratcikk
type: PeerReviewed
format: text
identifier: http://publicatio.bibl.u-szeged.hu/14980/1/1064793.pdf
identifier:     Áy Eva;  Hunyadi Attila;  Mezei Mária;  Minárovits János;  Hohmann Judit: Flavonol 7-O-Glucoside Herbacitrin Inhibits HIV-1 Replication through Simultaneous Integrase and Reverse Transcriptase Inhibition.   EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2019.  Paper 1064793-6 p.  ISSN 1741-427X (2019)     
identifier: doi:10.1155/2019/1064793
relation: 30609918
language: eng