TY - JOUR VL - 27 SP - 729 UR - http://publicatio.bibl.u-szeged.hu/10563/ JF - TETRAHEDRON-ASYMMETRY ID - publicatio10563 A1 - Galla Zsolt A1 - Forró Enik? A1 - Fülöp Ferenc AV - restricted EP - 731 N2 - A very efficient enzymatic two-step cascade reaction was devised (E > 200) for the resolution of activated gamma-lactams (+/-)-1 and (+/-)-2. The N-hydroxymethyl group worked as a traceless activating group, when the reactions were performed with H2O (0.5 equiv) in the presence of benzylamine (1 equiv) in i-Pr2O at 60 degrees C. The ring-opened enantiomerically pure gamma-amino acids (1S,4R)-6 (ee = 99%, intermediate of abacavir) and (1S,3R)-8 (ee = 99%) and unreacted lactams (1S,4R)-1 and (1R,4S)-2 (ee >= 96%) were obtained in good yields (>= 43%). Treatment of (1S,4R)-1 and (1R,4S)-2 with 18% HCl or NH4OH resulted in (1R,4S)-6 center dot HCl and (1S,3R)-8 center dot HCI or (15,4R)-3 and (1R,4S)-4 quantitatively, with ee >= 96%. (C) 2016 Elsevier Ltd. All rights reserved. IS - 16 N1 - FELTÖLT?: Nonn Melinda - nonn.melinda@pharm.u-szeged.hu TI - Enhanced enzymatic synthesis of the enantiopure intermediate for the blockbuster drug intermediate abacavir through a two-step enzymatic cascade reaction SN - 0957-4166 Y1 - 2016/// ER -